Overview
Course Description
The Tek-Z Genetic Engineering course covers modern biotechnological tools and genetic manipulation techniques used in research and healthcare. It’s ideal for biology students and professionals seeking a deep understanding of genetic innovation.
What you'll learn
- DNA, genes, and genetic code fundamentals
- Recombinant DNA technology
- Gene editing with CRISPR-Cas9
- Genetic cloning and sequencing methods
- Ethical implications in genetic engineering
Course Features:
- Research-based case studies
- Interactive video lessons
- Practical simulations
- Course completion certificate
Course Content
Introduction to genetic engineering: importance, tools, and applications
Career pathways in biotechnology, pharmaceuticals, agriculture, and forensics
Mendelian genetics: laws of inheritance and Punnett square analysis
Non-Mendelian inheritance: incomplete dominance, codominance, polygenic traits
Chromosomal inheritance: mitosis, meiosis, and genetic recombination
Pedigree analysis for genetic diseases
Live Project 1: Genetic trait prediction using Punnett squares and pedigree mapping
Structure and function of plasmids
Gene transfer methods: electroporation, microinjection,viral vectors, lipofection
Agrobacterium-mediated transformation in plants
PCR and gel electrophoresis – principles and applications
Blotting techniques (Southern, Northern, Western)
Mutations: types, causes, and engineering applications
Live Project 2: Gene amplification and visualization
using simulated PCR and electrophoresis
RNA interference and post-transcriptional gene silencing mechanisms
Applications of RNAi in disease therapy and crop improvement
CRISPR-Cas system: mechanism, components, and workflow
Applications of CRISPR in knockout, knock-in, and correction of genes
Case studies: CRISPR in genetic disease therapy and crop improvement
Ethical and regulatory concerns in gene editing
Live Project 3: CRISPR gene editing simulation and RNAi-based silencing model
Genetic modification of crops: yield, nutrition, and resistance
Transgenic plants and sustainable agriculture (Bt Cotton, Golden Rice)
Regulatory and public perception of GMOs
DNA fingerprinting and forensic genetics
PCR and STR analysis for genetic identification
Genetic screening and CRISPR-based diagnostics
Live Project 4: Design a GMO crop model and perform a virtual DNA fingerprint analysis
Development of therapeutic proteins and vaccines using genetic engineering
Recombinant DNA technology for biopharma applications
Gene therapy concepts and clinical case studies
CRISPR in developing mRNA vaccines and personalized medicine
Biosafety, clinical validation, and regulatory compliance
Live Project 5: Model a CRISPR-based therapeutic design workflow
Genomic databases: GenBank, NCBI, and UniProt for sequence retrieval
Sequence alignment and gene annotation tools
Use of AI in genomic prediction and drug discovery
Gene expression analysis using microarray data
Integration of bioinformatics with wet-lab genetic engineering
Live Project 6: Perform in-silico gene analysis using NCBI and alignment tools
Bioethics in genetic engineering and gene editing
Patent laws and regulatory frameworks (USFDA, DBT India)
GMO labeling and consumer rights
Genetic privacy and data protection in healthcare
Risk assessment and safe lab practices
Capstone Project: Design a genetic engineering experiment (crop, disease, or drug-based)
Interpretation of research data and result presentation
Preparing scientific reports and posters
Peer evaluation and feedback from mentors
Future of genetic engineering: AI, synthetic biology, and precision medicine